Contact an Account ManagerA direct injection method for determining furosemide and metolazone in urine using HPLC and fluorescence detection
Summary
Type: HPLC
Application #: 104970
Catalog#: 720120.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x150mm
Detection: fluorescence, excitation 272nm, emission 410nm for furosemide, excitation 245nm, emission 450nm for metolazone
Conditions: eluent: 10mM KH2PO4 adjusted to pH 3.0 with conc. phosphoric acid - acetonitrile (65:35, v/v)
flow rate 1.0ml/min for metolazone, 1.2ml/min for furosemide
Authors: Farthing D
Source: LC-GC Intl 4 No 11 (1991) 42-44
Details
Legend: Fig. 2. Chromatograms of (a) blank normal subject urine, (b) urine spiked with
25 ng/ml and (c) urine spiked with 1000ng/ml metolazone (A); (d) blank normal
subject urine, (e) urine spiked with 25ng